UNSAFE BIOTECHNOLOGY LABORATORY - NO ACCOUNTABILITY FOR INJURY - ILLNESS - DISEASE

WAS WORKING FOR A BIOTECH COMPANY SAFE? NOT WHEN ONE IS EXPOSED TO KNOWN AND UNKNOWN MICROORGANISMS. READ ABOUT THE EXPERIENCES OF WORKING FOR AGRAQUEST, INC. IN DAVIS - CALIFORNIA

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  • Proposed 2012 CLT Goals

    For the first part of the 2012 budget process, the CLT has come up with the following goals.

    Goal #1: Final Rollout of the New Site Templates

    Task: Rollout each new site template as they are ready

    Task: Open up a channel where people can provide feedback and report any usage issues

    Task: Respond to reported issues

    Target Date for Completion: As they are ready, between now and February 2012

    Resources required: Volunteer time.

    Goal #2: Assess the amount of data channels currently in use and try to condense where possible

    Task: Have a team of individuals from the CLT perform an analysis of the current community channels and see where some merging of resources and content can be done.

    Task: Combine research into a document to share with the CLT and request community feedback as well.

    Target Date for completion: February 2012

    Resources required: Volunteer time.

    Goal #3: Rollout Paid features for JRD/JED Directories.

    Task: Implement payment system

    Task: Define rules and guidelines, terms and conditions

    Task: Testing period before launch

    Target Date for completion: January 2012 for task #1 and #3. As soon as requested legal guidance is provided for Task #2

    Resources Required: Legal guidance and documentation, which needs to be provided through OSM. This has been requested, currently waiting for that information.

    Goal #4: Template Directory (JTD) Launch

    Task: Launch a working version of the Template Directory

    Task: Recruit a team of editors and put it to work

    Resources Required:

     

    • Legal guidance and documentation to determine that the Terms of Use for the site are aligned with our ™
    • Possible Travel for team members to meet face to face
    • Volunteer time is also required.

     

    Target Date for completion: February 2012

    We'd like to thank all of the volunteers on the various CWG teams who have helped to make the above goals possible for 2012. The Joomla community is lucky to have so many hard working people making these exciting things happen!

    We invite the community to provide feedback here in the CWG group of the Joomla People site: http://people.joomla.org/groups/viewdiscussion/1371-2012-proposed-clt-goals.html?groupid=398




  • Proposed PLT 2012 Goals

    As part of the normal budgeting process, the Production Leadership Team has come up with four goals for 2012. Those goals concern releases of the Joomla Platform and the Joomla CMS, continuing maintenance updates, and outreach and promotion to a technical audience.




  • Community feedback requested on OSM's top 2012 goal ideas

    As part of the Joomla! project's 2012 goal setting and budget planning proposed process, the Open Source Matters board of directors (OSM) has come up with some ideas for possible top OSM 2012 goals and priorities. Those ideas are listed below.




  • 2012 Goal setting and budget planning proposed process

    With an October 25th announcement on the public Joomla! leadership email list, work has begun on the Joomla! project's 2012 goal setting and budget planning proposed process. 2011 was the first year that the Production Leadership Team (PLT), Community Leadership Team, (CLT) and Open Source Matters board of directors (OSM) put in the extra effort to first define their goals for the year before they began work on their parts of the project's overall budget.




  • Roadmap Meeting Recap

    We'd like to thank everyone who participated in the experimental Joomla Roadmap Meeting that was held last Friday live in NYC and virtually via live streaming on Ustream and twitter. This meeting was looking at the next major revision of Joomla after the long-term support release 2.5 comes out in a few months.




FEATURED VIDEOS, AUDIOS, ARTICLES, EDITORIALS, BLOGS





marti.sandi.cssandra3.16.11show





martiSHOW3.23.2011

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*IS THE WORLD BEING  LED TO BELIEVE THAT ONLY "NATURAL" (naturally found in the environment)  BACTERIA AND/OR FUNGUS IS ONLY BEING USED IN MICROBIAL PRODUCTS; BIOPESTICIDES, BIOINSECTICIDES, BIOFUNGICIDES ETC. WHICH ARE BEING USED ON OUR FOOD CROPS, ORNAMENTALS AND/OR USED FOR INSECT CONTROL?   THIS IS NOT ALWAYS THE CASE... "MUTANTS" ARE BEING USED THAT THE PUBLIC IS NOT AWARE OF
Are we being led to believe these microbial products are safe??
*It seems that using "mutants" and mutants created using recombinant techniques. (last 2 patents below) doesn’t qualify the claim of "naturally found" bacteria and fungus which are claimed as being the “active ingredient” in microbial biocontrol products.
*When searching the United States Patent Office for the search terms of “Agraquest” and “mutants” the results were twenty (20) United States patents; that were assigned to Agraquest alone and furthermore does not take into account International patents. These patents uses the wording; mutants, mutants thereof and/or recombinant techniques.  There are many MANY more patents that don't list Agraquest as the holder of the patent; but Agraquest's scientists were listed as "inventors" on other company patents. (these are not listed below)
*The reader is invited; NO... encouraged to view the following twenty (20) Agraquest United States patents and decide for themselves whether we can be 100% certain that only “naturally found in the environment” bacteria and/or fungus is being used on our food crops, ornamentals and/or used for insect control.... OR are mutants being used?

READ MORE HERE


AUDIO:   "Falling To Be Held" fallingTObeHELD

Song: "Control" Listen → HERE

Applicable Lyrics:

You Can’t Control Me
You Know Me
I’ve had it up to here with your lies and your ties
~WHAT ABOUT MY LIFE ?~

PRESENTATION - FRAUD IN THE COURT - COMMITTED BY JUDGE SUZANNE F. DUGAN IN CALIFORNIA WORKERS COMPENSATION SYSTEM; by Sandi Trend

VIDEO - INJURED AGRAQUEST BIOTECH WORKER, DAVID BELL TELLS HIS STORY; by Labor Video Project

VIDEO - Workers Comp, The Destruction Of Ca-OSHA/EPA And The Case Of David Bell; by Labor Video Project

VIDEO - Conflict of Interest By Judge Says Sandi Trend, Mother of Injured Agraquest Biotech Worker Bell; by Labor Video Project

VIDEO - INJURED WORKERS AND ADVOCATES DEMAND THAT INSURANCE COMP FRAUD BE PROSECUTED; by Labor Video Project

VIDEO - 12/10/2009; SANDI TREND, MOTHER OF INJURED BIOTECH WORKER DAVID BELL ASKS CALIFORNIA COMMISSION ON HEALTH AND SAFETY & WORKERS COMPENSATION WHAT "GAMING THE SYSTEM IS?"; by Labor Video Project

VIDEO - Injured Worker, DAVID BELL's Mother Charges Fraud On 9/9/2009 At the California Department of Insurance; Fraud Assessment Comission Meeting; by Labor Video Project

VIDEO - JUNE 17, 2009 SANDI TREND SPEAKS OUT ABOUT FRAUD BY DISTRICT ATTORNEYS AT THE CALIFORNIA DEPARTMENT OF INSURANCE; FRAUD ASSESSMENT COMMISSION; by Labor Video Project

AUDIO - KDRT 95.7 PODCAST of Davis California's Journalist Interview - with David Bell, Doug Haney and Sandi Trend; by Davis, CA Journalist, David Greenwald *Yolo Judicial Watch)

ARTICLE - Biotech Workers Struggle For Safety Measures; by Seth Sandrosky: The Populist

ARTICLE - Biotech canaries - Sickened workers get little relief; by Seth Sandrosky: The Sacramento News & Review

ARTICLE - MARCH-APRIL 2010: COUNCIL FOR RESPONSIBLE GENETICS "GeneWatch MAGAZINE EDITORIAL on David Bell and Agraquest titled; TeaTime In The Lab; by GeneWatch Editor, Sam Anderson

ARTICLE - The Fungus and Bacteria of Deregulation and biotech Worker David Bell; by Steve Zeltzer - LaborNet.org

ARTICLE - Cal-Osha: Going Down The Tubes?; by Larry Rose MD, MPH Cal/OSHA Medical Unit

ARTICLE - The Last Physician/Medical Officer Position is Eliminated at Cal/OSHA; by Larry Rose MD, MPH Cal/OSHA Medical Unit

ARTICLE - Blood, phlegm and tears; by Seth Sandronsky - Sacramento News & Review

ARTICLE - The Criminal Cover-up Of Pam Marrone's Agraquest Operation; www.indybay.org/newsitems Central Valley | Labor & Workers

ARTICLE - Toxic Dump Sites And Agraquest/Pam Marrone Case May Get Light In Davis, California Hearing; by David Greenwald - Central Valley | Environment & Forest Defense | Health, Housing, and Public Services | Labor & Workers

ARTICLE - Local biotech employee says health affected by work.. Officials say no threat to public health; by California Aggie - Oooja Kumar

BLOG - Biotech Worker Safety; by JEEG, The Council for Responsible Genetics - GeneWatch

BLOG - Mother of Injured Biotech Worker Speaks Out On Conflicts Of Interest; by JEEG..."This could prove sufficient evidence to reopen the worker's compensation claim." by Council for Responsible Genetics

BLOG - MAN BECOMES INFECTED WHILE WORKING AT BIOTECH, AGRAQUEST; by WatchDog on Science

BLOG - Did Davis Biotech Firm Expose Davis to Potentially Dangerous Pathogens?; by The People's Vanguard of Davis

BLOG - California Aggie Covers Issue of Agraquest; Yolo County Health Discounts Health Concerns; by The People's Vanguard of Davis

BLOG - The Vanguard's Article on AgraQuest Provokes Strong Response From both County Health Director and Building Owner; by The People's Vanguard of Davis

BLOG - Did Congressman Lungren Ignore Potential National Security Threat Posed By AgraQuest's Imporation of Foreign Soils?; by The People's Vanguard of Davis

 


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Newsflash

Identification and Characterization of Novel Genetic Markers Associated with Biological Control Activities in Bacillus subtilis
-Raghavendra Joshi and Brian B. McSpadden Gardener Department of Plant Pathology, The Ohio State University, OARDC, 1680 Madison Ave., Wooster 44691.
Accepted for publication 3 October 2005.

PAGE 4:
RESULTS
Characterization of strain-specific DNA fragments. In order to identify DNA markers for biological control in B. subtilis, SSH was conducted to identify sequences present in the genomes of strains GB03 and QST713 but absent in the genome of strain 168. In all, 149 subtracted fragments (74 from GB03 and 75 from QST713) were cloned and analyzed. Fragment lengths varied from 180 to 1,520 bp, with a median fragment size of 560 bp. Se- quence analyses revealed that 80 of the subtracted fragments (36 from GB03 and 44 from QST713) had significant nucleotide homology (E value <1E-4) to previously identified Bacillus sequences. The sequences of these fragments can be found in GenBank under accession numbers DQ011366-401 (GB03) and DQ0113402-446 (QST713) in the same order as presented in Tables 1 and 2, respectively. In several instances, clones from both strains GB03 and QST713 were identified that had similarity to gene fragments from homologous operons. These included genes for the biosynthesis of cyclic lipopeptides (bam/bmy, fen, and srf ) and the cell wall component teichuronic acid (tua). Subtracted fragments matching the yndJ gene from B. subtilis subsp. amyloliquefaciens strain FZB42 also were recovered from both strains GB03 and QST713. Additionally, multiple sporulation gene fragments (spo) were found in both subtracted libraries.

DISCUSSION:
We characterized over 60,000 bp of genomic sequence obtained from strains of B. subtilis used in two different biocontrol prod- ucts. Nearly half of the 149 sequences we analyzed were sufficiently different from those presently in GenBank that no functional assignments could be made (data not shown). We expect that many of those unique sequences represent noncoding regions which are likely to be strain specific and therefore of limited value for population studies. Nonetheless, further characterization of these unique sequences is warranted because some may encode novel genes, an unknown fraction of which may be involved in biological control. Of the 80 Bacillus-like sequences we obtained, 65 were associated with genes predicted to encode a variety of metabolic functions. Much research has gone into establishing the function of B. subtilis genes, but such work has yet to reveal the function of all of the ORFs identified in strain 168 (48). In addition, their potential roles in bacterial fitness in the natural environment and biocontrol activities in a managed environment remain highly speculative. Nonetheless, several of these gene fragments identified present intriguing new avenues for investigating the nature of antibiotic-mediated biocontrol. For example, dppC is pre- dicted to code for dipeptide transporters and the homologs identi-

Nearly half of the 149 sequences we analyzed were sufficiently different from those presently in GenBank that no functional assignments could be made

Identification of subtracted genome fragments obtained from Bacillus subtilis QST713 [AGRAQUEST]

Bacillus amyloliquefaciens- RESULTS
Subtracted fragments matching the yndJ gene from B. subtilis subsp. amyloliquefaciens strain FZB42 also were recovered from both strains GB03 and QST713.
Bacillus  cereus - RESULTS
.....with the exception that one of two faint bands amplified from most B. cereus strains comigrated with the ituC product amplified from the positive control strain, QST713.
...And, interestingly enough, only some of the B. cereus isolates and B. pumilus GB34 were maximally antagonistic to Phytophthora sojae in these assays, though QST713 did show a marginal degree of inhibition in all assays (i.e., average score of 1.0).

Bacillus  licheniformis (page 7)
...However, similar types of associations were observed in other Bacillus spp. As a group, all of the B. pumilus and B. licheniformis isolates scored (weakly) positive for one or more gene markers and were more inhibitory to R. solani (P = 0.01) compared with those strains lacking the scored markers. Additionally, the two B. pumilus isolates that were scored weakly positive for the bmy gene (i.e., 1.1a2 and 2.5a) were significantly more inhibitory to Pythium ultimum than the other isolates lacking all of the markers (P = 0.04). However, presence of the amplifiable markers was not necessarily a good predictor of biocontrol capacity in B. pumilus because the commercialized biocontrol strain GB34 was scored negative for all of the amplifiable targets, yet displayed in vitro in- hibition capacities similar to those of the commercialized B. subtilis strains GB03, MBI600, and QST713.