UNSAFE BIOTECHNOLOGY LABORATORY - NO ACCOUNTABILITY FOR INJURY - ILLNESS - DISEASE
WAS WORKING FOR A BIOTECH COMPANY SAFE? NOT WHEN ONE IS EXPOSED TO KNOWN AND UNKNOWN MICROORGANISMS. READ ABOUT THE EXPERIENCES OF WORKING FOR AGRAQUEST, INC. IN DAVIS - CALIFORNIA
Agraquest/Biotech in Mexico
|
FEATURED VIDEOS, AUDIOS, ARTICLES, EDITORIALS, BLOGS
*IS THE WORLD BEING LED TO BELIEVE THAT ONLY "NATURAL" (naturally found in the environment) BACTERIA AND/OR FUNGUS IS ONLY BEING USED IN MICROBIAL PRODUCTS; BIOPESTICIDES, BIOINSECTICIDES, BIOFUNGICIDES ETC. WHICH ARE BEING USED ON OUR FOOD CROPS, ORNAMENTALS AND/OR USED FOR INSECT CONTROL? THIS IS NOT ALWAYS THE CASE... "MUTANTS" ARE BEING USED THAT THE PUBLIC IS NOT AWARE OF
*It seems that using "mutants" and mutants created using recombinant techniques. (last 2 patents below) doesn’t qualify the claim of "naturally found" bacteria and fungus which are claimed as being the “active ingredient” in microbial biocontrol products.
AUDIO: "Falling To Be Held" 
Song: "Control" Listen → HERE
Applicable Lyrics:
You Can’t Control Me
You Know Me
I’ve had it up to here with your lies and your ties
~WHAT ABOUT MY LIFE ?~
PRESENTATION - FRAUD IN THE COURT - COMMITTED BY JUDGE SUZANNE F. DUGAN IN CALIFORNIA WORKERS COMPENSATION SYSTEM; by Sandi Trend
VIDEO - INJURED AGRAQUEST BIOTECH WORKER, DAVID BELL TELLS HIS STORY; by Labor Video Project
VIDEO - Workers Comp, The Destruction Of Ca-OSHA/EPA And The Case Of David Bell; by Labor Video Project
VIDEO - Conflict of Interest By Judge Says Sandi Trend, Mother of Injured Agraquest Biotech Worker Bell; by Labor Video Project
VIDEO - INJURED WORKERS AND ADVOCATES DEMAND THAT INSURANCE COMP FRAUD BE PROSECUTED; by Labor Video Project
VIDEO - 12/10/2009; SANDI TREND, MOTHER OF INJURED BIOTECH WORKER DAVID BELL ASKS CALIFORNIA COMMISSION ON HEALTH AND SAFETY & WORKERS COMPENSATION WHAT "GAMING THE SYSTEM IS?"; by Labor Video Project
VIDEO - Injured Worker, DAVID BELL's Mother Charges Fraud On 9/9/2009 At the California Department of Insurance; Fraud Assessment Comission Meeting; by Labor Video Project
VIDEO - JUNE 17, 2009 SANDI TREND SPEAKS OUT ABOUT FRAUD BY DISTRICT ATTORNEYS AT THE CALIFORNIA DEPARTMENT OF INSURANCE; FRAUD ASSESSMENT COMMISSION; by Labor Video Project
ARTICLE - Biotech Workers Struggle For Safety Measures; by Seth Sandrosky: The Populist
ARTICLE - Biotech canaries - Sickened workers get little relief; by Seth Sandrosky: The Sacramento News & Review
ARTICLE - MARCH-APRIL 2010: COUNCIL FOR RESPONSIBLE GENETICS "GeneWatch MAGAZINE EDITORIAL on David Bell and Agraquest titled; TeaTime In The Lab; by GeneWatch Editor, Sam Anderson
ARTICLE - The Fungus and Bacteria of Deregulation and biotech Worker David Bell; by Steve Zeltzer - LaborNet.org
ARTICLE - Cal-Osha: Going Down The Tubes?; by Larry Rose MD, MPH Cal/OSHA Medical Unit
ARTICLE - The Last Physician/Medical Officer Position is Eliminated at Cal/OSHA; by Larry Rose MD, MPH Cal/OSHA Medical Unit
ARTICLE - Blood, phlegm and tears; by Seth Sandronsky - Sacramento News & Review
ARTICLE - The Criminal Cover-up Of Pam Marrone's Agraquest Operation; www.indybay.org/newsitems Central Valley | Labor & Workers
ARTICLE - Local biotech employee says health affected by work.. Officials say no threat to public health; by California Aggie - Oooja Kumar
BLOG - Biotech Worker Safety; by JEEG, The Council for Responsible Genetics - GeneWatch
BLOG - MAN BECOMES INFECTED WHILE WORKING AT BIOTECH, AGRAQUEST; by WatchDog on Science
Newsflash
|
Identification and Characterization of Novel Genetic Markers Associated with Biological Control Activities in Bacillus subtilis
-Raghavendra Joshi and Brian B. McSpadden Gardener Department of Plant Pathology, The Ohio State University, OARDC, 1680 Madison Ave., Wooster 44691.
Accepted for publication 3 October 2005.
PAGE 4:
RESULTS
Characterization of strain-specific DNA fragments. In order to identify DNA markers for biological control in B. subtilis, SSH was conducted to identify sequences present in the genomes of strains GB03 and QST713 but absent in the genome of strain 168. In all, 149 subtracted fragments (74 from GB03 and 75 from QST713) were cloned and analyzed. Fragment lengths varied from 180 to 1,520 bp, with a median fragment size of 560 bp. Se- quence analyses revealed that 80 of the subtracted fragments (36 from GB03 and 44 from QST713) had significant nucleotide homology (E value <1E-4) to previously identified Bacillus sequences. The sequences of these fragments can be found in GenBank under accession numbers DQ011366-401 (GB03) and DQ0113402-446 (QST713) in the same order as presented in Tables 1 and 2, respectively. In several instances, clones from both strains GB03 and QST713 were identified that had similarity to gene fragments from homologous operons. These included genes for the biosynthesis of cyclic lipopeptides (bam/bmy, fen, and srf ) and the cell wall component teichuronic acid (tua). Subtracted fragments matching the yndJ gene from B. subtilis subsp. amyloliquefaciens strain FZB42 also were recovered from both strains GB03 and QST713. Additionally, multiple sporulation gene fragments (spo) were found in both subtracted libraries. DISCUSSION:
We characterized over 60,000 bp of genomic sequence obtained from strains of B. subtilis used in two different biocontrol prod- ucts. Nearly half of the 149 sequences we analyzed were sufficiently different from those presently in GenBank that no functional assignments could be made (data not shown). We expect that many of those unique sequences represent noncoding regions which are likely to be strain specific and therefore of limited value for population studies. Nonetheless, further characterization of these unique sequences is warranted because some may encode novel genes, an unknown fraction of which may be involved in biological control. Of the 80 Bacillus-like sequences we obtained, 65 were associated with genes predicted to encode a variety of metabolic functions. Much research has gone into establishing the function of B. subtilis genes, but such work has yet to reveal the function of all of the ORFs identified in strain 168 (48). In addition, their potential roles in bacterial fitness in the natural environment and biocontrol activities in a managed environment remain highly speculative. Nonetheless, several of these gene fragments identified present intriguing new avenues for investigating the nature of antibiotic-mediated biocontrol. For example, dppC is pre- dicted to code for dipeptide transporters and the homologs identi- Nearly half of the 149 sequences we analyzed were sufficiently different from those presently in GenBank that no functional assignments could be made
Identification of subtracted genome fragments obtained from Bacillus subtilis QST713 [AGRAQUEST]
Bacillus amyloliquefaciens- RESULTS
Subtracted fragments matching the yndJ gene from B. subtilis subsp. amyloliquefaciens strain FZB42 also were recovered from both strains GB03 and QST713.
Bacillus cereus - RESULTS
.....with the exception that one of two faint bands amplified from most B. cereus strains comigrated with the ituC product amplified from the positive control strain, QST713.
...And, interestingly enough, only some of the B. cereus isolates and B. pumilus GB34 were maximally antagonistic to Phytophthora sojae in these assays, though QST713 did show a marginal degree of inhibition in all assays (i.e., average score of 1.0).
Bacillus licheniformis (page 7)
...However, similar types of associations were observed in other Bacillus spp. As a group, all of the B. pumilus and B. licheniformis isolates scored (weakly) positive for one or more gene markers and were more inhibitory to R. solani (P = 0.01) compared with those strains lacking the scored markers. Additionally, the two B. pumilus isolates that were scored weakly positive for the bmy gene (i.e., 1.1a2 and 2.5a) were significantly more inhibitory to Pythium ultimum than the other isolates lacking all of the markers (P = 0.04). However, presence of the amplifiable markers was not necessarily a good predictor of biocontrol capacity in B. pumilus because the commercialized biocontrol strain GB34 was scored negative for all of the amplifiable targets, yet displayed in vitro in- hibition capacities similar to those of the commercialized B. subtilis strains GB03, MBI600, and QST713. |
