UNSAFE BIOTECHNOLOGY LABORATORY - NO ACCOUNTABILITY FOR INJURY - ILLNESS - DISEASE

*Is the world being led to believe that only “natural” (naturally found in the environment)  bacteria and/or fungus is only being used in microbial products; biopesticides, bioinsecticides, biofungicides etc. which are being used on food crops, ornamentals and/or used for insect control? Are we being led to believe these microbial products are safe; when in fact they are not?
*It seems that using "mutants" and mutants created using recombinant techniques. (last 2 patents below) doesn’t qualify the claim of "naturally found" bacteria and fungus which are claimed as being the “active ingredient” in microbial biocontrol products.
*When searching the United States Patent Office for the search terms of “Agraquest” and “mutants” the results were twenty (20) United States patents; that were assigned to Agraquest alone and furthermore does not take into account International patents. These patents uses the wording; mutants, mutants thereof and/or recombinant techniques.  There are many MANY more patents that don't list Agraquest as the holder of the patent; but Agraquest's scientists were listed as "inventors" on other company patents. (these are not listed below) 
*The reader is invited; NO... encouraged to view the following twenty (20) United States patents and decide for themselves whether we can be 100% certain that only “naturally found in the environment” bacteria and/or fungus is being used on our food crops, ornamentals and/or used for insect control.... OR are mutants being used?
*www.biotechawareness.com received a confirmation from a 20+ year molecular biologist which states:
You are right.  It is a big cover-up.  Yes, there are naturally occurring mutants that evolve through evolution.  But the production of mutants through chemical or recombinant DNA techniques are not the same. They are not naturally occurring and instead are man-induced or man-made.  Using microbial biocontrol as a form of remediation or pesticide application is not necessarily safe even when using naturally occurring microbes.

USPTO PATENT SEARCH FOR "mutants " AND AN/agraquest

( 1 of 20 ) United States Patent 7,754,203
Strobel ,   et al.
July 13, 2010
Endophytic fungi and methods of use
•The term "mutant" refers to a variant of the parental strain as well as methods for obtaining a mutant or variant in which the desired biological activity is similar to that expressed by the parental strain. The "parent strain" is defined herein as the original Muscodor strains before mutagenesis. Mutants occur in nature without the intervention of man. They also are obtainable by treatment with or by a variety of methods and compositions known to those of skill in the art. For example, parental strains may be treated with a chemical such as N-methyl-N'-nitro-N-nitrosoguanidine, ethylmethanesulfone, or by irradiation using gamma, x-ray, or UV-irradiation, or by other means well known to those practiced in the art.•Thus, this invention provides an isolated novel fungi designated Muscodor and two species thereof, Muscodor albus and Muscodor roseus, and mutants thereof.

Lehman ,   et al.
February 8, 2005
Metabolite from streptomyces strain NRRL accession No. B-30145 and mutants thereof for controlling plant diseases
What is claimed is:
•1. A composition comprising at least one isolated metabolite produced by a biologically pure Streptomyces sp. strain selected from a Streptomyces sp. strain having all the identifying characteristics of the strain deposited with NRRL with Accession No. B-30145; and mutants of the strain deposited with NRRL with Accession No. B-30145, wherein the mutants have all the identifying characteristics of NRRL No. B-30145; and wherein the metabolite exhibits activity against plant pathogenic fungi, shows UV absorption between about 215 nm and about 220 nm and is not aromatic.
•12. An antifungal composition comprising the composition of claim 1 wherein the metabolite is isolated according to a method comprising: (a) loading a whole broth culture of Streptomyces sp. strain NRRL No. B-30145 or mutants thereof that have all the identifying characteristics of NRRL No. B-30145 onto a non-ionic absorbent polymeric resin; (b) eluting the metabolite with an alcohol; (c) screening the eluent of step (b) with a bioassay for fractions of the eluent exhibiting antifungal activity; (d) loading the fractions of the eluent exhibiting antifungal activity of step (c) on a HPLC column; and (e) eluting the metabolite with an organic solvent, to produce the antifungal composition.
DETAILED DESCRIPTION
•The present invention provides a novel strain of Streptomyces sp. or mutants thereof with antifungal activity only on specific plant pathogens such as Alternaria, Phytophthora, Botrytis, Rhizoctonia and Sclerotinia. This novel strain was deposited with the NRRL on Jul. 20, 1999 under the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure under Accession No. B-30145.
•The term "mutant" refers to a variant of the parental strain as well as methods for obtaining a mutant or variant in which the pesticidal activity is greater than that expressed by the parental strain. The "parent strain" is defined herein as the original Streptomyces strain before mutagenesis. To obtain such mutants the parental strain may be treated with a chemical such as N-methyl-N'-nitro-N-nitrosoguanidine, ethylmethanesulfone, or by irradiation using gamma, x-ray, or UV-irradiation, or by other means well known to those practiced in the art.
•We describe a novel antibiotic-producing strain of Streptomyces sp. NRRL No. B-30145 and mutants thereof that have antifungal activity only on specific plant pathogens. Also provided is a supernatant isolated from the culture as well as a composition comprising the culture. In a further aspect, the compositions further comprise at least one chemical or biological pesticide.
•(a) loading a whole broth culture of Streptomyces sp. strain NRRL No. B-30145 or mutants thereof having all the identifying characteristics of NRRL No. B-30145

Lehman ,   et al.
January 27, 2004
Streptomyces strain with insecticidal activity and method of using as an insecticide
We claim:
•1. An isolated Streptomyces galbus strain selected from the group consisting of a Streptomyces galbus strain having all the identifying characteristics of the Streptomyces galbus, strain deposited with NRRL as Accession No. 30232, variants of the strain deposited with NRRL as Accession No. 30232, wherein the variants have all the identifying characteristics of the NRRL No. 30232 strain; and mutants of the strain deposited with NRRL as Accession No. 30232, wherein the mutants have all the identifying characteristics of the NRRL No. 30232 strain;
DESCRIPTION OF THE INVENTION
•The present invention provides a novel compound, Streptomyces galbus, NRRL Accession No. 30232, and mutants thereof which retain the same activity, for use as an insecticide against Lepidoptera.
•The term "mutant" refers to a variant of the parental strain as well as methods for obtaining a mutant or variant in which the pesticidal activity is greater than that expressed by the parental strain. The "parent strain" is defined herein as the original Streptomyces strain before mutagenesis. To obtain such mutants  the parental strain may be treated with a chemical such as N-methyl-N'-nitro-N-nitrosoguanidine, ethylmethanesulfone, or by irradiation using gamma, x-ray, or UV-irradiation, or by other means well known to those practiced in the art.
•This invention provides a biologically pure culture of a strain having all the identifying characteristics of Streptomyces galbus, NRRL Accession No. 30232, and its variants and mutants , as described above, that have insecticidal activity. In one embodiment, the invention is the strain designated NRRL Accession No. 30232.
•The isolated supernatants have insecticidal activity similar to that of a Streptomyces galbus, NRRL Accession No. 30232, or mutants  and variants thereof.

( 4 of 20 ) United States Patent 6,638,910
Heins ,   et al.
October 28, 2003
Compositions and methods for controlling plant pests
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain, AQ713, of Bacillus subtilis, previously identified as a Bacillus megaterium, or mutants  thereof with the broad antifungal, insecticidal and antibacterial activity. This novel strain is designated AQ713 and was deposited with the NRRL on Mar. 7, 1997 under the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure under Accession No. B21661. It was subsequently identified as Bacillus subtilis by the American Type Culture Collection (ATCC).
•A "mutant" is an organism bearing a mutant gene that expresses itself in the phenotype of the organism.

Lehman ,   et al.
October 21, 2003
Strain of bacillus for controlling plant diseases

What is claimed is:
•1. A composition for treating a fungal infection in a plant, root or fruit comprising a combination of: a) an effective amount of a whole broth culture of a strain having all the identifying characteristics of a Bacillus pumilus strain designated NRRL Accession No. B-30087, or mutants thereof that have fungicidal activity and b) an effective amount of a whole broth culture of a strain having all the identifying characteristics of a Bacillus subtilis strain designated NRRL Accession No. B-21661, or mutants  thereof that have fungicidal activity.
•8. A composition for treating a fungal infection in a plant, root or fruit comprising a combination of: a) an effective amount of a whole broth culture of a strain having all the identifying characteristics of a Bacillus pumilus strain designated NRRL Accession No. B-30087, or mutants  thereof that have fungicidal activity and b) an effective amount of a whole broth culture of a strain having all the identifying characteristics of a Bacillus subtilis strain designated NRRL Accession No. B-21661, or mutants  thereof that have fungicidal activity, wherein the combination of (a) and (b) has synergistic fungicidal activity when combined in a ratio of 2:1.
•12. A composition for treating a fungal infection in a plant, root or fruit comprising a combination of: a) an effective amount of a whole broth culture of a strain having all the identifying characteristics of a Bacillus pumilus strain designated NRRL Accession No. B-30087, or mutants  thereof that have fungicidal activity and b) an effective amount of a whole broth culture of a strain having all the identifying characteristics of a Bacillus subtilis strain designated NRRL Accession No. B-21661, or mutant thereof that have fungicidal activity, wherein the combination of (a) and (b) has synergistic fingicidal activity when combined in a ratio of about 4:1.
MODES FOR CARRYING OUT THE INVENTION
•The present invention provides a biologically pure culture of a strain having all the identifying characteristics of a novel strain of Bacillus sp. and mutants  or variants thereof, with antifungal activity only on specific plant pathogens such as rusts, powdery mildews and downy mildews. This novel strain of B. pumilus was deposited on Jan. 14, 1999,
•We describe a biologically pure culture of a strain having all the identifying characteristics of a novel antibiotic-producing strain of Bacillus sp. deposited under NRRL Accession No. B-30087, and mutants  thereof, that have antifungal activity only on specific plant pathogens and no antibacterial activity. In one aspect, the strain is Bacillus pumilus deposited under NRRL Accession No. B-30087, and mutants  of the strain.
•In other aspects, the strain is a mutant or variant of NRRL Accession No. B-30087 that has all the identifying characteristics (as provided below) of the strain deposited under NRRL Accession No. B-30087. mutant or variant are used interchangeably throughout this disclosure and further, may be identified as having a genome that hybridizes under conditions of high stringency to the genome of NRRL Accession No. B-30087. "Hybridization" refers to a reaction in which one or more polynucleotides react to form a complex that is stabilized via hydrogen bonding between the bases of the nucleotide residues. The hydrogen bonding may occur by Watson-Crick base pairing, Hoogstein binding, or in any other sequence-specific manner. The complex may comprise two strands forming a duplex structure, three or more strands forming a multi-stranded complex, a single self-hybridizing strand, or any combination of these. Hybridization reactions can be performed under conditions of different "stringency." In general, a low stringency hybridization reaction is carried out at about 40.degree. C. in 10.times.SSC or a solution of equivalent ionic strength/temperature. A moderate stringency hybridization is typically performed at about 50.degree. C. in 6.times.SSC, and a high stringency hybridization reaction is generally performed at about 60.degree. C. in 1.times.SSC.
•A mutant or variant of NRRL Accession No. B-30087 may also be defined as a strain having a genomic sequence that is greater than 85%, more preferably greater than 90% or more preferably greater than 95% sequence identity to the genome of NRRL Accession No. B-30087.
•Further provided by this invention are compositions comprising any of the above strains (including mutants or variants thereof), supernatants, fractions and metabolites, alone or in combination with each other, and a carrier. These compositions may be further supplemented by the addition of at least one chemical or biological pesticide. These compositions may take the form of various formulations, which include, but are not limited to, a wettable powder, a granule formulation, an aqueous suspension, an emulsifiable concentrate or microencapsulation.

Lehman ,   et al.
July 1, 2003
Strain of bacillus for controlling plant diseases
What is claimed is:
•1. A method of treating a fungal infection in a plant, root or fruit comprising applying an effective amount of a strain having all the identifying characteristics of a Bacillus pumilus strain designated NRRL Accession No. B-30087, or mutants  thereof that have fungicidal activity, to the plant, root or fruit.
•5. A method of treating a fungal infection in a plant, root or fruit comprising applying an effective amount of a composition comprising a strain having all the identifying characteristics of Bacillus pumilus strain designated NRRL Accession No. B-30087, or mutants thereof that have fungicidal activity and a carrier, to the plant, root or fruit.
MODES FOR CARRYING OUT THE INVENTION
•The present invention provides a biologically pure culture of a strain having all the identifying characteristics of a novel strain of Bacillus sp. or mutants  thereof with antifungal activity only on specific plant pathogens such as rusts, powdery mildews and downy mildews. This novel strain is deposited with the NRRL on Jan. 14, 1999 under the provisions of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purpose of Patent Procedure under Accession No. B-30087.
•We describe a biologically pure culture of a strain having all the identifying characteristics of a novel antibiotic-producing strain of Bacillus sp. NRRL No. B-30087, and mutants  thereof, that have antifungal activity only on specific plant pathogens and no antibacterial activity. In one aspect, the strain is Bacillus pumilis deposited under NRRL No. B-30087, and mutants of the strain.
•Further provided by this invention are compositions comprising any of the above strains (including mutants  or variants thereof), supernatants, and metabolites, alone or in combination with each other, and a carrier. These compositions may be further supplemented by the addition of at least one chemical or biological pesticide.

Lehman ,   et al.
February 25, 2003
Strain of Streptomyces for controlling plant diseases
What is claimed is:
•1. An isolated, biologically pure culture of Streptomyces sp. strain NRRL No. B-30145 and mutants  thereof having all of the identifying characteristics of said strain NRRL No. B-30145.
MODES FOR CARRYING OUT THE INVENTION
•The present invention provides a novel strain of Streptomyces sp. or mutants  thereof with antifungal activity only on specific plant pathogens such as Alternaria, Phytophthora, Botrytis, Rhizoctonia and Sclerotinia. This novel strain was deposited with the NRRL on Jul. 20, 1999
•The term "mutant" refers to a variant of the parental strain as well as methods for obtaining a mutant or variant in which the pesticidal activity is greater than that expressed by the parental strain. The "parent strain" is defined herein as the original Streptomyces strain before mutagenesis. To obtain such mutants  the parental strain may be treated with a chemical such as N-methyl-N'-nitro-N-nitrosoguanidine, ethylmethanesulfone, or by irradiation using gamma, x-ray, or UV-irradiation, or by other means well known to those practiced in the art.
•We describe a novel antibiotic-producing strain of Streptomyces sp. NRRL No. B-30145 and mutants  thereof that have antifungal activity only on specific plant pathogens. Also provided is a supernatant isolated from the culture as well as a composition comprising the culture. In a further aspect, the compositions further comprise at least one chemical or biological pesticide.

Heins ,   et al.
July 9, 2002
Compositions and methods for controlling plant pests
What is claimed is:
•1. A method for protecting or treating plants or fruit from insect infestations comprising applying a composition comprising an effective amount of a lipopeptide extract isolated from Bacillus subtilis strain NRRL B-21661 and mutants  thereof having insecticidal activity to plants or fruit in need thereof.
•2. A method for protecting or treating plants or fruit from insect infestations comprising applying a composition comprising an effective amount of a surfactin lipopeptide isolated from Bacillus subtilis strain NRRL B-21661 and mutant thereof having insecticidal activity to plants or fruit in need thereof.
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain, AQ713, of Bacillus subtilis, previously identified as a Bacillus megaterium, or mutants  thereof with the broad antifungal, insecticidal and antibacterial activity. This novel strain is designated AQ713 and was deposited with the NRRL on Mar. 7, 1997
•A "mutant" is an organism bearing a mutant gene that expresses itself in the phenotype of the organism.

Heins ,   et al.
September 18, 2001
Strain of bacillus for controlling plant diseases and corn rootworm
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain, AQ713, of Bacillus subtilis, previously identified as a Bacillus megaterium, or mutants  thereof with the broad antifungal and antibacterial activity and the novel combination of antifungal and anti-corn rootworm activity. This novel strain is designated AQ713 and was deposited with the NRRL on Mar. 7, 1997

( 10 of 20 ) United States Patent 6,245,551
Lehman ,   et al.
June 12, 2001
Strain of Bacillus pumilus for controlling plant diseases caused by fungi
What is claimed is:
•1. A biologically pure culture of a strain having all the identifying characteristics of a Bacillus pumilus strain designated NRRL No. B-30087, or mutants  thereof, that have fungicidal activity.
MODES FOR CARRYING OUT THE INVENTION
•The present invention provides a biologically pure culture of a strain having all the identifying characteristics of a novel strain of Bacillus sp. or mutants  thereof with antifungal activity only on specific plant pathogens such as rusts, powdery mildews and downy mildews. This novel strain is deposited with the NRRL on Jan. 14, 1999
•We describe a biologically pure culture of a strain having all the identifying characteristics of a novel antibiotic-producing strain of Bacillus sp. NRRL No. B-30087, and mutants  thereof, that have antifungal activity only on specific plant pathogens and no antibacterial activity. In one aspect, the strain is Bacillus pumilis deposited under NRRL No. B-30087, and mutants  of the strain.
•Further provided by this invention are compositions comprising any of the above strains (including mutants  or variants thereof), supernatants, and metabolites, alone or in combination with each other, and a carrier. These compositions may be further supplemented by the addition of at least one chemical or biological pesticide

Heins ,   et al.
April 3, 2001
Bacillus mycoides strain for controlling insects
What is claimed is:
•1. A supernatant obtained from an isolated, pure culture of Bacillus mycoides strain AQ726, or its mutants  and variants, that has insecticidal activity.
•3. A metabolite produced by the Bacillus mycoides strain AQ726, NRRL Accesion No. B21664 or mutants  and variants thereof, that exhibits insecticical activity, is water soluble and has a molecular weight of less than 10,000 daltons.
•4. A composition comprising the Bacillus mycoides strain AQ726, NRRL Accesion No. B21664 or mutants  and variants thereof, that exhibits pesticidal activity.
•5. A method for protecting or treating plants from insect infestations comprising applying to the plant or its environment an effective amount of the Bacillus mycoides AQ726, NRRL Accesion No. B21664 or mutants  and variants thereof.
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain of Bacillus mycoides and mutants  and variants thereof that produces a metabolite that exhibits pesticidal activity. The novel strain is designated Bacillus mycoides AQ726 and was deposited with the NRRL on Mar. 7, 1997

( 12 of 20 ) United States Patent 6,103,228
Heins ,   et al.
August 15, 2000
Compositions and methods for controlling plant pests
What is claimed is:
•1. A method for protecting or treating plants and fruit from insect infestations, comprising applying an effective amount of the Bacillus subtilis strain AQ713, NRRL Accession No. B-21661, or mutants  thereof having all the identifying characteristics of said strain, to said plants and fruit.
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain, AQ713, of Bacillus subtilis, previously identified as a Bacillus megaterium, or mutants  thereof with the broad antifungal, insecticidal and antibacterial activity. This novel strain is designated AQ713 and was deposited with the NRRL on Mar. 7, 1997
•A "mutant" is an organism bearing a mutant gene that expresses itself in the phenotype of the organism.

Marrone ,   et al.
June 20, 2000
Strain of bacillus for controlling plant disease
What is claimed is:
•1. An isolated, pure culture of Bacillus thuringiensis strain AQ52, NRRL Accession No. B-21619 and mutants  thereof, said pure culture and mutants having pesticidal activity against fungal or bacterial infections.

(14 of 20 ) United States Patent 6,060,051
Heins ,   et al.
May 9, 2000
Strain of bacillus for controlling plant diseases and corn rootworm
What is claimed is:
•1. A biologically pure culture of Bacillus subtilis strain AQ713, NRRL Accession No. B21661 and mutants  thereof having all the identifying characteristics of said strain.
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain, AQ713, of Bacillus subtilis, previously identified as a Bacillus megaterium, or mutants  thereof with the broad antifungal and antibacterial activity and the novel combination of antifungal and anti-corn rootworm activity. This novel strain is designated AQ713 and was deposited with the NRRL on Mar. 7, 1997

( 15 of 20 ) United States Patent 6,027,723
Heins ,   et al.
February 22, 2000
Rhodococcus globerulus strain for controlling corn rootworm
What is claimed is :
•1. A biologically pure culture of a strain of Rhodococcus having all the identifying characteristics of Rhodococcus globerulus strain AQ719, NRRL Accession No. B21663 or mutants  thereof,
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain of Rhodococcus globerulus and mutants  and variants thereof that produces a metabolite that exhibits pesticidal activity against corn rootworms, nematodes and beet armyworms. The novel strain is designated Rhodococcus globerulus AQ719 and was deposited with the NRRL on Mar. 7, 1997

( 16 of 20 ) United States Patent 6,015,553
Germida ,   et al.
January 18, 2000
Bacillus subtilis strain for controlling insect and nematode pests
What is claimed is:
•1. An isolated, pure culture of Bacillus strain AQ743, NRRL Accession No. B-21665, having pesticidal activity, and mutants  thereof, which mutants  maintain the pesticidal activity.
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain of Bacillus subtilis isolated from wheat roots or mutants  and variants thereof that produce a metabolite with anti-corn rootworm activity. This novel strain is designated AQ743, and was deposited with the Agriculture Research Culture Collection (NRRL) having a place of business at 1815 N. University Street, Peoria, Ill. 610604, U.S.A., on Mar. 7, 1997

( 17 of 20 ) United States Patent 6,001,637
Heins ,   et al.
December 14, 1999
Bacillus pumilus strain for controlling corn rootworm, nematode and armyworm infestations
What is claimed is:
•1. A biologically pure culture of a strain of Bacillus having all the identifying characteristics of Bacillus pumilus stain AQ717, NRRL Accession No. B21662 or mutants  thereof,
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain of Bacillus pumilus and mutants  and variants thereof that produces a metabolite that exhibits pesticidal activity against corn rootworms, nematodes and beet armyworms. The novel strain is designated Bacillus pumilus AQ717 and was deposited with the NRRL on Mar. 7, 1997

( 18 of 20 ) United States Patent 5,906,818
Heins ,   et al.
May 25, 1999
Bacillus mycoides strain for controlling corn rootworm
What is claimed is :
•1. An isolated, pure culture of Bacillus mycoides strain AQ726, NRRL Accession No. B21664 and its mutants  and variants.
MODES OF CARRYING OUT THE INVENTION
•The present invention provides a novel strain of Bacillus mycoides and mutants  and variants thereof that produces a metabolite that exhibits pesticidal activity against corn rootworms. The novel strain is designated Bacillus mycoides AQ726 and was deposited with the NRRL on Mar. 7, 1997

( 19 of 20 ) United States Patent 5,733,544
Marrone ,   et al.
March 31, 1998
Nematicidal bacillus strain and metabolite and methods of use thereof
What is claimed is:
•1. An isolated, pure culture of a novel strain of Bacillus chitinosporus, designated AQ746, NRRL Accession Number B-21618 or mutants  thereof which maintain all of the identical characteristics of the deposited strain, which strain secretes a toxic metabolite having nematicidal and insecticidal activity.
•3. An isolated soluble toxic metabolite obtained from the centrifuged supernatant of a culture of Bacillus chitinosporus, designated AQ746, NRRL Accession Number B-21618 or mutants  thereof which maintain all of the identical characteristics of the deposited strain, such that said toxic metabolite has nematicidal and insecticidal activity.
•An isolated, pure culture of a novel Bacillus chitinosporus strain AQ746 and mutants and variants thereof exhibiting nematicidal activity are provided. Also provided is a nematicidal and insecticidal metabolite obtained from the supernatant of a culture of this novel strain.
•Another aspect of the invention provides mutants or variants of the AQ746 strain which retain nematicidal and insecticidal activity and produce a nematicidal and insecticidal metabolite in their supernatant. Such mutants or variants have altered genotypic or phenotypic characteristics, and may be produced by genetic manipulation techniques well known in the art.
•Examples of genetic mutants include, but are not limited to, mutants selected for phenotype by classical genetic methods and mutants created using recombinant techniques.

Chilcott ,   et al.
July 8, 1997
Bacillus thuringiensis strain and metabolite which are active against corn rootworm
We claim :
•1. An isolated, pure culture of a novel strain of Bacillus thuringiensis BD#32, NRRL B-21530, or mutants thereof that maintain all the identifying characteristics of the deposited strain,
SUMMARY OF THE INVENTION
•An isolated, pure culture of a novel Bacillus thuringiensis strain BD#32 or mutants thereof exhibiting insecticidal activity is provided.
•Another aspect of the invention provides mutants or derivatives of the BD#32 strain which retain insecticidal activity and produce insecticidal agents in their supernatant. Such mutants or derivatives have altered genotypic or phenotypic characteristics, and may be produced by genetic manipulation techniques known in the art.
•Examples of genetic mutants include, but are not limited to, mutants selected for phenotype by classical genetic methods and mutants created using recombinant techniques.